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Study on the transport of humic acids through the plant cuticles
Smilková, Marcela ; Záhora, Jaroslav (referee) ; Sedláček, Petr (advisor)
This diploma thesis is focused on foliar fertilization. It is nowadays one of the most widespread types of application of commercial fertilizers. Concern of this thesis is the study of plant cuticles as a thin layer on the leaves. These thin membranes are responsible for regulation of water transport and nutrients. Further function of plant cuticles is the mechanical protection of the outer part of the plant. Plant cuticles were characterized by fluorescence spectroscopy, classical optical microscopy and profilometry. The main aim of this thesis is the optimization and verification of experimental methodology aimed to the transport of commercial humic product through plant cuticles by simple diffusion techniques. The results obtained by presented diffusion techniques of humic product through plant cuticles were correlated with the same diffusion experiments through synthetic membranes with defined pore size and density. The study on transport of commercial humic product was realized in hydrogel medium by two different diffusion methods – non-stationary diffusion technique and diffusion couple. The important part of this diploma thesis is the characterization of supported hydrogel matrix by rheology tests, mercury intrusion porosimetry and scanned electron microscopy. Conclusions of this diploma thesis could be used for improving of efficiency of foliar fertilization.
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Usage of physical-chemical methods for the study of interaction of humic substances with the plant cutisles
Rampáčková, Eliška ; Sedláček, Petr (referee) ; Smilková, Marcela (advisor)
This bachelor’s thesis deals with the study of interaction (or binding or adsorption) of plant cuticle with potassium lignohumate, as a liquid agriculture product used in the field of foliar fertilization, which belongs to the most used agronomic applications in general. The studied materials became the plant cuticle, which forms upper part of plants and potassium lignohumate (commercially Lignohumate), which is widely used thanks to its properties, which allow to increase the quality, growth and profitability of the crops. The aim of this bachelor’s thesis was to investigate the binding of compounds to plant cuticles, useful methods for this field of research and the use of thermal methods (precisely isothermal microcalorimetry and thermogravimetry). The next step was to use this instrumentation as a physicochemical method for assessing the ability of Lignohumate to bind to plant cuticles.
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Study on transport of liquid lignohumates through plant leaves and plant cuticles
Rampáčková, Eliška ; Weidlich, Tomáš (referee) ; Smilek, Jiří (advisor)
This thesis deals with the study of transport commercial solution of pottasium lignohumate through the plant cuticles, as the main barriers in transport of active ingredience to plant tissues. The problematics has been studied to obtain the informations about ability of transport liquid active ingredience during foliar fertilization, which became popular in theme of efective utilization fertilizers and reduction their negative impact to enviroment. The aim of this thesis was to optimize technics of diffusion for demanded purposes in the Stokes diffusion cells. As the transport barriers have been used the plant cuticles, that have been isolated by various methods. The ultraviolet and visible spectrometry (UV-VIS) was the analytical method for determination of transported volume of lignohumate and to the mentioned experiments the cuticles of Prunus laurocerasus and cuticles of tomato fruit have been used. The isolated cuticles have been analyzed by optical microscopy, scanning electron microscopy and profilometry. The characterisation of the used solution of lignohumate was also important part of this thesis. It has been used the basic physico-chemical methods and also the advanced methods as tensiometry, size exclusion chromatography with UV detector and differential refractive index detector (SEC-UV-dRI) and thermogravimetry (TGA).
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Study on transport of liquid lignohumates through plant leaves and plant cuticles
Rampáčková, Eliška ; Weidlich, Tomáš (referee) ; Smilek, Jiří (advisor)
This thesis deals with the study of transport commercial solution of pottasium lignohumate through the plant cuticles, as the main barriers in transport of active ingredience to plant tissues. The problematics has been studied to obtain the informations about ability of transport liquid active ingredience during foliar fertilization, which became popular in theme of efective utilization fertilizers and reduction their negative impact to enviroment. The aim of this thesis was to optimize technics of diffusion for demanded purposes in the Stokes diffusion cells. As the transport barriers have been used the plant cuticles, that have been isolated by various methods. The ultraviolet and visible spectrometry (UV-VIS) was the analytical method for determination of transported volume of lignohumate and to the mentioned experiments the cuticles of Prunus laurocerasus and cuticles of tomato fruit have been used. The isolated cuticles have been analyzed by optical microscopy, scanning electron microscopy and profilometry. The characterisation of the used solution of lignohumate was also important part of this thesis. It has been used the basic physico-chemical methods and also the advanced methods as tensiometry, size exclusion chromatography with UV detector and differential refractive index detector (SEC-UV-dRI) and thermogravimetry (TGA).
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|
|
Usage of physical-chemical methods for the study of interaction of humic substances with the plant cutisles
Rampáčková, Eliška ; Sedláček, Petr (referee) ; Smilková, Marcela (advisor)
This bachelor’s thesis deals with the study of interaction (or binding or adsorption) of plant cuticle with potassium lignohumate, as a liquid agriculture product used in the field of foliar fertilization, which belongs to the most used agronomic applications in general. The studied materials became the plant cuticle, which forms upper part of plants and potassium lignohumate (commercially Lignohumate), which is widely used thanks to its properties, which allow to increase the quality, growth and profitability of the crops. The aim of this bachelor’s thesis was to investigate the binding of compounds to plant cuticles, useful methods for this field of research and the use of thermal methods (precisely isothermal microcalorimetry and thermogravimetry). The next step was to use this instrumentation as a physicochemical method for assessing the ability of Lignohumate to bind to plant cuticles.
|
|
|
Study on the transport of humic acids through the plant cuticles
Smilková, Marcela ; Záhora, Jaroslav (referee) ; Sedláček, Petr (advisor)
This diploma thesis is focused on foliar fertilization. It is nowadays one of the most widespread types of application of commercial fertilizers. Concern of this thesis is the study of plant cuticles as a thin layer on the leaves. These thin membranes are responsible for regulation of water transport and nutrients. Further function of plant cuticles is the mechanical protection of the outer part of the plant. Plant cuticles were characterized by fluorescence spectroscopy, classical optical microscopy and profilometry. The main aim of this thesis is the optimization and verification of experimental methodology aimed to the transport of commercial humic product through plant cuticles by simple diffusion techniques. The results obtained by presented diffusion techniques of humic product through plant cuticles were correlated with the same diffusion experiments through synthetic membranes with defined pore size and density. The study on transport of commercial humic product was realized in hydrogel medium by two different diffusion methods – non-stationary diffusion technique and diffusion couple. The important part of this diploma thesis is the characterization of supported hydrogel matrix by rheology tests, mercury intrusion porosimetry and scanned electron microscopy. Conclusions of this diploma thesis could be used for improving of efficiency of foliar fertilization.
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